Methods & Protocols

Optical Estimation of Bioelectric Patterns in Living Embryos
McMillen, P., and Levin, M. (2024)
in M. Bizzarri (Ed.), Systems Biology: Methods in Molecular Biology, 2745: 91-102, doi:10.1007/978-1-0716-3577-3_6

Optimization of Oligomer Stamping Technique for Normally Closed Elastomeric Valves on Glass Substrate
Dungan, J., Mathews, J., Levin, M., and Koomson, V. (2023)
Micromachines, 14(9): 1659

Information Theory as an experimental tool for integrating disparate biophysical signaling modules
McMillen, P., Walker, S. I., and Levin, M. (2022)
International Journal of Molecular Sciences, 23(17): 9580

Screening Biophysical Sensors and Neurite Outgrowth Actuators in Human Induced-Pluripotent-Stem-Cell-Derived Neurons
Pai, V. P., Cooper, B. G., and Levin, M. (2022)
Cells, 11(16): 2470

Studying Protista WBR and Repair Using Physarum polycephalum
Sperry, M. M., Murugan, N. J., and Levin, M. (2022)
in S. Blanchoud and B. Galliot (Eds.), Whole-Body Regeneration: Methods in Molecular Biology, 2450: 51-67

Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model
Nanos, V., and Levin, M. (2021)
in M. R. Ebrahimkhani and J. Hislop (Eds.), Programmed Morphogenesis: Methods in Molecular Biology, 2258: 93-103

Toward Decoding Bioelectric Events in Xenopus Embryogenesis: New Methodology for Tracking Interplay between Calcium and Resting Potentials In Vivo
McMillen, P., Novak, R., and Levin, M. (2020)
Journal of Molecular Biology, 432(2): 605-620

Defined extracellular ionic solutions to study and manipulate the cellular resting membrane potential
Bonzanni, M., Payne, S. L., Adelfio, M., Kaplan, D. L., Levin, M., and Oudin, M. J. (2020)
Biology Open, 9(1): bio048553

Live Imaging of Intracellular pH in Planarians Using the Ratiometric Fluorescent Dye SNARF-5F-AM
Beane, W. S., Adams, D. S., Morokuma, J., and Levin, M. (2019)
Biology Methods and Protocols, 4(1): bpz005

Inverse Drug Screening of Bioelectric Signaling and Neurotransmitter Roles: Illustrated Using a Xenopus Tail Regeneration Assay
Sullivan, K. G., and Levin, M. (2018)
Cold Spring Harbor Protocols, 2018(3): 154-163

A Tunable Silk Hydrogel Device for Studying Limb Regeneration in Adult Xenopus laevis
Golding, A., Guay, J. A., Herrera-Rincon, C., Levin, M., and Kaplan, D. L. (2016)
PLoS One, 11(6): e0155618

Automatic neuron segmentation and neural network analysis method for phase contrast microscopy images
Pang, J., Özkucur, N., Ren, M., Kaplan, D. L., Levin, M., and Miller, E. L. (2015)
Biomedical Optics Express, 6(11): 4395-4416

Optogenetics in developmental biology: using light to control ion flux-dependent signals in Xenopus embryos
Adams, D.S., Lemire, J. M., Kramer, R. H., and Levin, M. (2014)
International Journal of Developmental Biology, 58(10-12): 851-861

Humane anesthesia and pain management in amphibian limb surgery of Rana pipiens
Koustubhan, P., Kaplan, D., and Levin, M. (2013)
Cold Spring Harbor Protocols, 2013(2): 149-155

Light-activated serotonin for exploring its action in biological systems
Rea, A. C., Vandenberg, L. N., Ball, R. E., Snouffer, A. A., Hudson, A. G., Zhu, Y., McLain, D. E., Johnston, L. L., Lauderdale, J. D., Levin, M., and Dore, T. M. (2013)
Chemistry & Biology, 20(12): 1536-1546

Photoconversion for Tracking the Dynamics of Cell Movement in Xenopus laevis Embryos
Chernet, B. T., Adams, D. S., and Levin, M. (2012)
Cold Spring Harbor Protocols, 2016(6): 683-690

A versatile protocol for mRNA electroporation of Xenopus laevis embryos
Chernet, B. T., and Levin, M. (2012)
Cold Spring Harbor Protocols, 2012(4): 447-452

General principles for measuring resting membrane potential and ion concentration using fluorescent bioelectricity reporters
Adams, D. S., and Levin, M. (2012)
Cold Spring Harbor Protocols, 2012(4): 385-397

Measuring resting membrane potential using the fluorescent voltage reporters DiBAC4(3) and CC2-DMPE
Adams, D. S., and Levin, M. (2012)
CSHL Protocols, doi: 10.1101/pdb.prot067702

Aversive training methods in Xenopus laevis: general principles
Blackiston, D. J., and Levin, M. (2012)
Cold Spring Harbor Protocols, 2012(5): 521-527

Patterned femtosecond-laser ablation of Xenopus laevis melanocytes for studies of cell migration, wound repair, and developmental processes
Mondia, J. P., Adams, D. S., Orendorff, R. D., Levin, M., and Omenetto, F. (2011)
Biomedical Optics Express, 2(8): 2383-2391

High-throughput Xenopus laevis immunohistochemistry using agarose sections
Blackiston, D., Vandenberg, L. N., and Levin, M. (2010) ​​​​
Cold Spring Harbor Protocols, 2010(12): 1364-1371

Live imaging of planarian membrane potential using DiBAC4(3)
Oviedo, N. J., Nicolas, C. L., Adams, D. S., and Levin, M. (2008)
Cold Spring Harbor Protocols, 3(10): pdb.prot5055

Gene knockdown in planarians using RNA interference
Oviedo, N. J., Nicolas, C. L., Adams, D. S., and Levin, M. (2008)
Cold Spring Harbor Protocols, 3(10): pdb.prot5054

Establishing and maintaining a colony of planarians
Oviedo, N. J., Nicolas, C. L., Adams, D. S., and Levin, M. (2008) ​​​​
Cold Spring Harbor Protocols, 3(10): pdb.prot5053

Analysis of behavior in the planarian model
Nicolas, C. L., Abramson, C. I., and Levin, M. (2008)
in R. B. Raffa & S. M. Rawls (Eds.), Planaria: A Model for Drug Action and Abuse, RG Landes Co.: Austin, pp. 83-94

Establishing and maintaining a Xenopus laevis colony for research laboratories
Koustubhan, P., Sorocco, D., and Levin, M. (2007)
in M. Conn (Ed.), Source Book of Models for Biomedical Research, Humana Press, pp. 139-160

Inverse Drug Screens: a rapid and inexpensive method for implicating molecular targets
Adams, D. S., and Levin, M. (2006) ​​​​
Genesis, 44(11): 530-540

Strategies and techniques for investigation of biophysical signals in patterning
Adams, D., and Levin, M. (2006)
in M. Whitman and A. K. Sater (Eds.), Analysis of Growth Factor signaling in Embryos. Methods in Signal Transduction Series, CRC Press: Boca Raton FL, pp. 177-264

A novel immunohistochemical method for evaluation of antibody specificity and detection of labile targets in biological tissue
Levin, M. (2004)
Journal of Biophysical and Biochemical Methods, 58(1): 85-96

Use of Genetic Algorithms to Solve Biomedical Problems
Levin, M. (1995)
M.D. Computing, 12(3): 193-198